Axonal transport of ribonucleoprotein particles (vaults).

作者: J.-Y. Li , W. Volknandt , A. Dahlstrom , C. Herrmann , J. Blasi

DOI: 10.1016/S0306-4522(98)00622-8

关键词:

摘要: RNA was previously shown to be transported into both dendritic and axonal compartments of nerve cells, presumably involving a ribonucleoprotein particle. In order reveal potential mechanisms transport we investigated the major vault protein electric ray Torpedo marmorata. This is component particle (vault) carrying non-translatable has wide distribution in animal kingdom. It highly enriched cholinergic electromotor neurons similar size synaptic vesicles. The vaults by immunofluorescence, using anti-vault antibody as marker, cytofluorimetric scanning, compared that vesicle membrane SV2 beta-subunit F1-ATPase marker for mitochondria. Following crush significant accumulation proximal could first observed after 1 h, mitochondria 3 h 6 although weekly fluorescent traces accumulations were confocal microscope early h. Within time-period (up 72 h) all markers increased continuously. Retrograde also occurred, immunofluorescence retrograde component, indicating recycling, weaker than anterograde suggesting more half are degraded within terminal. High resolution revealed granular structure-in accordance with biochemical characteristics vaults. Of interest observation increase immunoreactivity accelerated time crushing, while SV2-containing particles appeared decelerate, procedure may have induced perikaryal alterations production subsequent export axon vesicles protein. Our data show ribonucleoprotein-immunoreactive can actively axons situ from soma terminal back. results suggest driven fast motors like Vaults exhibit an vesicular organelles Although function still unknown studies this organelle insights cellular general.

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