Detection of tick-borne pathogens in ticks from dogs and cats in different European countries

摘要: Abstract Ticks are known to transmit pathogens which threaten the health and welfare of companion animals man globally. In present study, mainly adult ticks were collected from dogs cats presented at their local veterinary practice in Hungary, France, Italy, Belgium (dogs only) Germany (cats only), identified based on tick morphology. If more than one was a host animal, pooled by species for DNA extraction subsequent PCR examination presence tick-borne pathogens. Out 448 samples, 247 (95 152 cats) Ixodes ricinus, 26 (12 14 I. hexagonus, 59 (43 16 Dermacentor reticulatus 116 (74 42 Rhipicephalus sanguineus sensu lato (s.l.). 17% ricinus samples Anaplasma phagocytophilum found. Borrelia spp. (18%) lesser extent dog-sourced (1%), with afzelii (n = 11), B. garinii (n = 7), valaisiana (n = 5), lusitaniae (n = 3) burgdorferi stricto being identified. One hexagonus sample cat France tested positive afzelii. Babesia canis detected 20% D. Hungary. s.l. found Hepatozoon (3%), A. platys (5%) three Rickettsia (7%; R. massiliae; raoultii rhipicephali). Furthermore, total 66 subjected molecular analysis as sp. II-temperate lineage, seven haplotypes recorded. Amongst them, most prevalent sequence types haplotype XIII (n = 24; 69%) XIV (n = 16; 52%) respectively, both dogs. Although differences related country host, observed, results this study indicate that exposed pathogen infected ticks, may represent medical risk these animals.