The SLT2 mitogen-activated protein kinase-mediated signalling pathway governs conidiation, morphogenesis, fungal virulence and production of toxin and melanin in the tangerine pathotype of Alternaria alternata.
作者： JONAR INGAN YAGO , CHING-HSUAN LIN , KUANG-REN CHUNG
DOI: 10.1111/J.1364-3703.2010.00701.X
关键词:
摘要: SUMMARY Fungi respond and adapt to different environmental stimuli via signal transduction systems. We determined the function of a yeast SLT2 mitogen-activated protein (MAP) kinase homologue (AaSLT2) in Alternaria alternata, fungal pathogen citrus. Analysis loss-of-function mutant indicated that AaSLT2 is required for production host-selective toxin, crucial pathogenicity. Moreover, A. alternata slt2 mutants displayed hypersensitivity cell wall-degrading enzymes chemicals such as Calcofluor white Congo red. This implicates an important role maintenance wall integrity A. alternata. The were also hypersensitive heteroaromatic compound, 2-chloro-5-hydroxypyridine, plant growth regulator, 2,3,5-triiodobenzoic acid. Developmentally, gene product was shown be critical conidial formation hyphal elongation. Compared with wild-type, produced fewer but slightly larger conidia less transverse septae. accumulated lower levels melanin chitin. Unlike wild-type progenitor, globose, swollen hyphae did not elongate straight radial direction. All defective phenotypes restored by transformation expression copy under control its endogenous promoter. study highlights MAP kinase-mediated signalling pathway, regulating diverse physiological, developmental pathological functions, tangerine pathotype
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