The use of highly sensitive and stable radionuclide embedded gold nanoparticles for in vivo imaging of macrophage migration to acute inflammation

摘要: 1143 Objectives Herein, we sought to evaluate the feasibility of highly sensitive and stable radionuclide embedded gold nanoparticles as a cell tracker for nuclear medicine imaging by monitoring macrophage migration toward chemically induced acute inflammatory lesions in mice well visualizing effects anti-inflammatory drugs migration. Methods Previously, have developed vivo platform, which is called RIe-AuNPs. After labeling macrophages with RIe-AuNPs, proliferation, phenotype marker expression, phagocytic activity were determined. Labeled intravenously administered to inflammation was generated injection 1% carrageenan (CG) solution footpad on day 1 post-transfer labeled macrophages, followed PET/CT biodistribution study. To examine effect drug migration, single dose dexamethasone (DEX) intraperitoneally administered immediately after induction monitored PET/CT. Results The RIe-AuNPs did not alter expression activity. In revealed selective labeled macrophages lesion early 3 h post-transfer, peak at 6 slight decline radioactive signal 24 h. The bio-distribution study results also consistent data. Inhibition inflamed DEX treatment successfully Conclusions We only tracked using but evaluated inhibitory DEX sites, suggest that tracking strategy will be useful research areas macrophage-related disease cell-based therapy.