Interactions between copy number and expression level of genes involved in fluconazole resistance in Candida glabrata.
作者： Salma Abbes , Charles Mary , Hayet Sellami , Annie Michel-Nguyen , Ali Ayadi
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摘要: Objectives. This study aimed to elucidate the relative involvement of drug resistance gene copy number and overexpression in fluconazole clinical C. glabrata isolates using a population-based approach. Methods. Fluconazole levels were quantified minimal inhibitory concentration (MIC) via Etest method. Both expression CgCDR1, CgPDH1, CgERG11 CgSNQ2 assessed quantitative real-time PCR. The influence main effects first-level interactions both level these genes on analyzed multivariate statistical model. Results. Forty-three collected from 30 patients during hospital survey. In analysis, MICs independently increased by (p
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