Purification of the low density lipoprotein receptor, an acidic glycoprotein of 164,000 molecular weight.
作者： W J Schneider , U Beisiegel , J L Goldstein , M S Brown
DOI: 10.1016/S0021-9258(18)34975-5
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摘要: This paper describes a rapid two-step procedure for the purification of low density lipoprotein receptor from bovine adrenal cortex membranes. After solubilization with nonionic detergents, adheres tightly to DEAE-cellulose column at pH 6. Following elution DEAE-cellulose, detergent is removed, leaving in soluble form. The then subjected affinity chromatography on coupled Sepharose 4B. eluted suramin, newly-found inhibitor lipoprotein-receptor interactions. yields single protein molecular weight 164,000. same also isolated when crude fraction applied an immunoaffinity containing monoclonal antibody directed against receptor. 164,000-dalton has acidic isoelectric point 4.6, which rises 4.8 after extensive treatment neuraminidase. purified retains all binding properties intact cells and
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