Production of Cloned Pigs from Adult Somatic Cells by Chemically Assisted Removal of Maternal Chromosomes

作者: Yin XiJun Yin XiJun , T Tani , I Yonemura , M Kawakami , K Miyamoto

DOI: 10.1095/BIOLREPROD67.2.442

关键词:

摘要: The present study demonstrated that brief treatment of in vitro-matured porcine oocytes with demecolcine results a membrane protrusion contains condensed chromosome mass, which can be easily removed by aspiration. This simple, chemically assisted method for removing maternal chromosomes enabled the production large number nuclear-transferred eggs. development eggs whose were this procedure following transfer somatic cell nuclei to blastocyst stage was not significantly different among groups activated using procedures (6% 11%) and also donor cells origins (3% 9%), except cumulus (0.4%). After 180 341 received 6 recipients, 2 recipients produced 8 healthy cloned piglets from heart female pig. effective bovine rabbit

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