Conserved MicroRNA Act Boldly During Sprout Development and Quality Formation in Pingyang Tezaocha (Camellia sinensis).

摘要: Tea tree [Camellia sinensis (L.) O. Kuntze] is an important leaf (sometimes tender stem)-using commercial plant with many medicinal uses. The development of newly sprouts would directly affect the yield and quality tea product, especially significant for Pingyang Tezaocha (PYTZ) which takes up a large percent in early spring market. MicroRNA (miRNA), particularly conserved miRNAs, often position center subtle complex gene regulatory systems, precisely control biological processes together other factors spatio-temporal pattern. Here, quality-determined metabolites catechins, theanine caffeine PYTZ including buds (sBud), different stages leaves (sL1, sL2) stems (sS1, sS2) were quantified. A total 15 miRNA libraries same tissue three repetitions each constructed to explore vital miRNAs during formation. We analyzed whole profiles sprout defined families plant. differentially expressed related expression buds, leaves, described. Twenty one eight miRNA-TF pairs that most likely participate regulating development, at least two miRNA-TF-metabolite triplets both formation had been filtered. Our results indicated act boldly processes, they are (i) more be linked morphological function primary metabolism (ii) hold secondary plant, also (iii) coordinate transcription forming networks multicellular organism regulation.