Antigen presentation is enhanced by targeting antigen to the Fc epsilon RII by antigen-anti-Fc epsilon RII conjugates.

摘要: Targeting Ag to the Fc epsilon RII by Ag-specific IgE has been shown be an efficient means of enhancing presentation B cells T cells. To take advantage as a targeting molecule and investigate whether was required for mediation enhanced stimulation, covalently coupled anti-Fc using heterobifunctional crosslinking reagents. These Ag-Ab conjugates were used with cell lines specific Ags, OVA (BB6.5) or rabbit gamma-globulin (CDC35 D1.6), splenic examine both proliferation in vitro. Significant Ag-anti-Fc apparent at doses 1,000- 10,000-fold lower than seen unconjugated alone. use RII-Ag good better Ab adhesion Pgp-1 control those assays (10- 100-fold). Anti-Fc clearly more effectively presented gamma (> Mouse is presently known expressed on follicular dendritic these vitro results suggest that would useful tools investigating role IgE-mediated vivo. BALB/c mice immunized OVA-anti-Fc made quite significant OVA-specific IgG1 response detectable response. No produced alone minimal when isotype-matched conjugate used. Thus, indicate operative vivo