Human p53 oncogene contains one promoter upstream of exon 1 and a second, stronger promoter within intron 1

摘要: Abstract To gain insight into how transcription of the human p53 oncogene is controlled, we characterized regulatory regions gene. A 3.8-kilobase-pair (kbp) EcoRI restriction fragment encompassing 5' end gene, as well subfragments generated by digests, was cloned upstream Escherichia coli chloramphenicol acetyltransferase (CAT) gene and CAT activity assayed in extracts transfected cells. Two types vectors were used: Epstein-Barr virus oriP-derived constructs that stably introduced cell lines K562, Raji, HL-60, pSV0-CAT-derived transiently monkey line COS. By this approach have identified two promoters for One promoter, p53P1, located 100-250 bp 218-bp noncoding first exon; a second, stronger p53P2, maps within intron. expression RNA indicate p53P2 functions up to 50-fold more efficiently than p53P1. We conclude may be controlled differential regulation these play an important role altered both normal transformed