Scavenger receptors on liver Kupffer cells mediate the in vivo uptake of oxidatively damaged red blood cells in mice.

摘要: In vitro studies have shown that damaged red cells and apoptotic are efficiently phagocytosed by scavenger receptors from macrophages, even under non-opsonizing conditions. Damaged blood in vivo effectively removed the circulation, but responsible receptor systems largely unknown. We used a murine model which (51)Cr-labeled oxidized were injected intravenously, cellular uptake sites potential involvement of analyzed. The decay was rapid (more than 50% within 10 minutes after injection), whereas native not cleared. main site liver Kupffer cells, contained 24% dose at injection. inhibited typical ligands for receptors, such as polyinosinic acid, liposomes containing phosphatidylserine, low-density lipoprotein, fucoidan, polyadenosinic acid or without phosphatidylserine. Mice lacking class A type I II showed no significant decrease ability to take up circulation. conclude mainly removal process mediated acid- phosphatidylserine-sensitive different II. Our data indicate pattern-recognizing play an important role apoptotic, damaged, other unwanted (Blood. 2000;95:2157-2163)