Selenite and Selenate Inhibit Human Lymphocyte Growth via Different Mechanisms
作者： Mikael Björnstedt , Giannis Spyrou , Arne Holmgren , Sven Skog
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摘要: Selenium compounds like selenite and selenate have strong inhibitory effects, particularly on mammalian tumor cell growth by unknown mechanisms. We found that the addition of sodium inhibited human 3B6 BL41 lymphocytes. Selenite was more potent because 10 microM produced a effect similar to 250 selenate. The mechanism action appears be different. cells treated with accumulated in S-phase; however, caused an accumulation G2. Selenite-mediated inhibition irreversible, although effects could reversed. Selenite, contrast selenate, is efficiently reduced thioredoxin system (thioredoxin, reductase, NADPH). At concentrations required observe growth, activity recently shown selenoprotein, increased selenite-treated decreased selenate-treated cells. Ribonucleotide reductase vitro assay selenodiglutathione but not These results show use different mechanisms inhibit growth.
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