Immunoglobulin G antibodies to Helicobacter pylori in patients with dyspeptic symptoms investigated by the western immunoblot technique.

摘要: Abstract Helicobacter pylori is a gram-negative, curved, rod-shaped bacterium known to cause gastritis and be an important factor in the pathogenesis of peptic ulcers. Serological testing has recently been proposed as aid diagnosis H. infections. In this study, we used Western immunoblot technique evaluate possibility using one or more antigens from for purpose. Thirteen major bands about 30 minor could identified by blotting when sera 53 consecutive dyspeptic patients, 27 healthy children, 25 blood donors were evaluated. Antibodies against most found significantly frequently patients with infections than without such However, antibodies single polypeptide not produced all infection. Polypeptides molecular masses 120, 50, between 19 36 kDa seem specific polypeptides This study showed only differences antigenic composition different clinical isolates pylori, serological cross-reactions other bacteria limited. Major Campylobacter jejuni bacterial lipopolysaccharide. band at 60 reacted antiserum Legionella micdadei common antigen, which may indicate cross-reaction antigen several bacteria. demonstrates that number useful tests after isolation purification.