Anaesthesia and analgesia for surgery in rabbits and rats : A comparison of the effects of different compounds

摘要: Studies of anaesthesia and analgesia in rats rabbit were undertaken with the aim to improve anesthesia techniques, for better animal welfare research quality. Induction volatile halogenated agents isoflurane, sevoflurane desflurane was compared New Zealand White rabbit. All caused struggling, breath holding reflex bradycardia, having least detrimental effects. Hypoxia prevented by pre-oxygenation, no cardiac arrhythmias seen. Still, induction rabbits cannot be recommended any these agents. Injection ketamine (cyclohexamine)/medetomidine (alpha-2-adrenergic agonist) effect adding opioid butorphanol, evaluated Additionally, effects after subcutaneous intramuscular administration ketamine/medetomidine compared. In a first study, dose 15/0.25 mg/kg found effective producing surgical 59 ± 18 min, but failed produce some animals following possibly due different stress levels at time induction. The anesthetic did not differ between routes. Subcutaneous injection easier perform seemed less painful. Pronounced hypoxia developed during (PaO2 4.8 0.6 kPa, mean SD), indicating need oxygen supplementation. Blind tracheal intubation easy anaesthesia. Addition butorphanol increased duration Wistar rats, ketamine/medetomidine, repeated six times weekly intervals, pre-medication buprenorphine, evaluated. Buprenorphine an increase as well greater respiratory depression, associated lethality. Repeated without buprenorphine safe led sleep successive anaesthetics. combination sufentanil (opioid)/medetomidine also rats. Anaesthesia more efficiently produced than intraperitoneal administration, sc 40/150 g/kg sufentanil/medetomidine 101 49 min. resulted very low saturation (40 20 %). Despite this, all recovered uneventfully. Oxygen supplementation is strongly this combination. Aanaesthesia reversed within 7 min 0.2/0.5 (mixed -opioid agonist/antagonist)/atipamezole (alphaadrenergic antagonist). Postoperative recovery behavior Sprague-Dawley abdominal surgery under isoflurane or anaesthesia, perioperative treatment NSAID analgesic carprofen studied. Surprisingly, body weight faster showed pain-related when performed locomotion reduced. on just seen immediate postoperative period, several days after. Perioperative reduced both anaesthetized animals. results show that choice may important use improved reduction pain surgery. LIST OF PUBLICATIONS I. Flecknell PA, Roughan JV, Hedenqvist P. Laboratory Animals, 1999, 33(1): 41-6. II. P, Antunes L, Orr H, PA. 2001, 35(2): 172-9. III. Assessment Rabbit. HE, LM. Veterinary Analgesia, 28, 18-25. IV. rabbit: influence route butorphanol. LM, 2002, 29, 14-19. V. Effects pre-anaesthetic 2000, 34(2):207-11. VI. Sufentanil medetomidine rat its reversal atipamezole 34(3): 244-51. VII. anaesthetic related behaviour development splenectomy rat. Backhans A, Brodin E. Manuscript.