ARID4B Knockdown Suppresses PI3K/AKT Signaling and Induces Apoptosis in Human Glioma Cells

摘要: Purpose Glioblastoma multiforme is a highly malignant primary brain cancer with poor prognosis. We recently reported that ARID4B could potentially serve as biomarker associated survival in glioma patients. However, the function of human gliomas remains unclear. The aim this study to investigate molecular cell biology role cells. Materials and Methods Gene Expression Omnibus (GEO) Human Protein Atlas (HPA) datasets were analyzed for expression WHO pathological grading, overall immunohistochemical staining. Using quantitative RT-PCR Western blotting, those findings confirmed normal tissue lines. knockdown was conducted via lentivirus-based transfection small hairpin RNA cells proliferation, cycle, apoptosis. Results In present study, our analysis GEO showed mRNA higher grade IV tumors (n = 81) than non-tumor control 23, P <0.0001). suppressed proliferation induced G1 phase arrest PI3K/AKT pathway. It also increased HDAC1, leading acetyl-p53 acetyl-H3 levels reduced migration invasion. These effects mediated downregulation AKT pathway components, including p-mTOR, p-PI3K p-AKT. led Cyclin D1, which apoptosis Conclusion correlates positively pathologic grading glioma. suppresses signaling induces results suggests acts an oncogene gliomas.