Biotransformation of 4-hydroxyphenylacetonitrile to 4-hydroxyphenylacetic acid using whole cell arylacetonitrilase of Alcaligenes faecalis MTCC 12629

摘要: Abstract The whole cell arylacetonitrilase of Alcaligenes faecalis MTCC 12629 was employed to develop a bioprocess for the synthesis 4-hydroxyphenylacetic acid (4-HPAA) from 4-hydroxyphenylacetonitrile (4-HPAN). Isobutyronitrile emerged as most appropriate inducer with 1.2-fold enhanced nitrilase production in feeding approach. A. preferably hydrolyzed 4-HPAN, 4-aminoyphenylacetonitrile, mandelonitrile and phenylacetonitrile their corresponding acids. Thermostability profile revealed stability at 40, 45 50°C half-life 45 h, 36 h 13 h, respectively. Michaelis constant (Km) reaction velocity (Vmax) were calculated 20 mM 2 U/mg dcw, This exhibited complete conversion 4-HPAN 4-HPAA 45°C 50 mM K2HPO4/KH2PO4 buffer (pH 7.0) 30 min. A fed batch process designed 500 ml five feedings substrate resulted 150 mM product formation using 18 U/ml cells (16 mg/ml dcw) 45°C. is first report on through mediated pathway volumetric catalytic productivity 23 g/l 4.13 g/g dcw/h,