Selective recognition of ds-DNA cavities by a molecular rotor: switched fluorescence of thioflavin T

摘要: Thioflavin T (ThT) has been widely utilized as a fluorescent marker for amyloid fibrils. However, the use of ThT an efficient reporter specific DNA structure still remains in question. Here, we report that fluorescence intensity is obviously enhancement when it binds to ds-DNAs which contain cavity structures such abasic site, gap site or mismatch site. Such cannot be achieved without these structures. The cavities provide appropriate spaces accommodate and allow occurrence some interactions. stacking interaction bound with context bases main driving force binding cavities. This restricts excited state's rapid torsional rotation around single C–C bond between benzothiazole dimethylaminobenzene moieties thus results decreased population nonradiative twisted internal charge-transfer (TICT) state. It impossible this occur property can used recognize high selectivity sensitivity. We expect ability target potential developed into practical functional biomaterials sensors devices.