Recovering DNA and optimizing PCR conditions from microdissected formalin-fixed and paraffin-embedded materials.
作者： Zhi-Ping Ren , Jan Sällström , Christer Sundström , Monica Nistér , Yngve Olsson
DOI: 10.1159/000055926
关键词:
摘要: Microdissection is a powerful technique in molecular pathology and genetic investigations. To detect alterations such as gene mutation or deletion from tumor specimen, the purity of target cel
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Uhlén M, Pontén J, Lundeberg J, Pontén F, Ren Zp, Ahmadian A, Hedrum A, Nistér M, Human epidermal cancer and accompanying precursors have identical p53 mutations different from p53 mutations in adjacent areas of clonally expanded non-neoplastic keratinocytes. Oncogene. ,vol. 12, pp. 765- 773 ,(1996)
A Hedrum, F Pontén, Z Ren, J Lundeberg, J Pontén, M Uhlén, Sequence-based analysis of the human p53 gene based on microdissection of tumor biopsy samples. BioTechniques. ,vol. 17, pp. 118- 129 ,(1994)
M. Uhlén, J. Pontén, J. Lundeberg, Z. P. Ren, A. Ahmadian, F. Pontén, Cecilia Berg, M. Nistér, Benign clonal keratinocyte patches with p53 mutations show no genetic link to synchronous squamous cell precancer or cancer in human skin. American Journal of Pathology. ,vol. 150, pp. 1791- 1803 ,(1997)
M.Mitchell Smith, Histone structure and function. Current Opinion in Cell Biology. ,vol. 3, pp. 429- 437 ,(1991) , 10.1016/0955-0674(91)90070-F
T. J. Richmond, J. T. Finch, B. Rushton, D. Rhodes, A. Klug, Structure of the nucleosome core particle at 7 Å resolution Nature. ,vol. 311, pp. 532- 537 ,(1984) , 10.1038/311532A0
S Hahn*, XY Zhong, C Troeger, R Burgemeister, K Gloning, W Holzgreve, Current applications of single-cell PCR. Cellular and Molecular Life Sciences. ,vol. 57, pp. 96- 105 ,(2000) , 10.1007/S000180050501
D. E. Wells, Compilation analysis of histones and histone genes. Nucleic Acids Research. ,vol. 14, ,(1986) , 10.1093/NAR/14.SUPPL.R119
Michael Grunstein, Histones as regulators of genes. Scientific American. ,vol. 267, pp. 68- 74 ,(1992) , 10.1038/SCIENTIFICAMERICAN1092-68
L. Schermelleh, S. Thalhammer, W. Heckl, H. Pösl, T. Cremer, K. Schütze, M. Cremer, Laser microdissection and laser pressure catapulting for the generation of chromosome-specific paint probes. BioTechniques. ,vol. 27, pp. 362- 367 ,(1999) , 10.2144/99272RR04
Karin Schüitze, Georgia Lahr, Identification of expressed genes by laser-mediated manipulation of single cells Nature Biotechnology. ,vol. 16, pp. 737- 742 ,(1998) , 10.1038/NBT0898-737