The application of the bioelectric recognition assay for the detection of human and plant viruses: definition of operational parameters
作者： S Kintzios , E Pistola , J Konstas , F Bem , Th Matakiadis
DOI: 10.1016/S0956-5663(01)00161-0
关键词:
摘要: The bioelectric recognition assay (BERA) is a novel biosensory method based on unique combination of group cells, their immobilization in matrix that preserves physiological functions and the expression cell interaction with viruses as change electrical properties. A BERA sensor consists an electroconductive, tube-like probe containing components immobilized cells gel matrix. Cells are selected to specifically interact virus under detection. In this way, when positive sample added probe, characteristic, 'signature-like' potential occurs upon contact between present study, we demonstrate can be used for detection humans (hepatitis C virus) plants (tobacco cucumber viruses) remarkably specific, rapid (1-2 min), reproducible cost-efficient fashion. sensitivity (0.1 ng) equal or even better than advanced immunological, cytological molecular techniques, such reverse transcription polymerase chain reaction. Moreover, good storability sensors achieved without affecting performance. use portable biosensors medicine, mass screening purposes, well biological warfare agents prior knowledge specific receptor-molecule discussed.
elsevier.com
sci-hub.se 参考文章(35)
F Garcia Jr, F Garcia, C Roldan, I Lopez, NM Martinez, M Alvarez, MC Bernal, J Hernandez, MC Maroto, Detection of HCV and GBV-CHGV RNA in peripheral blood mononuclear cells of patients with chronic type C hepatitis. Microbios. ,vol. 103, pp. 7- 15 ,(2000)
Kent Jardemark, Owe Orwar, Detection of biologically active molecules by use of pre-activated cell-based biosensors in liquid-based separation systems ,(1998)
Howard M. Shapiro, Cytological assay procedure ,(1979)
M Iwata, S Komori, T Unno, N Minamoto, H Ohashi, Modification of membrane currents in mouse neuroblastoma cells following infection with rabies virus. British Journal of Pharmacology. ,vol. 126, pp. 1691- 1698 ,(1999) , 10.1038/SJ.BJP.0702473
P Fromherz, A Offenhausser, T Vetter, J Weis, A neuron-silicon junction: a Retzius cell of the leech on an insulated-gate field-effect transistor. Science. ,vol. 252, pp. 1290- 1293 ,(1991) , 10.1126/SCIENCE.1925540
Stuart A. MacFarlane, Rapid cloning of uncharacterised tobacco rattle virus isolates using long template (LT) PCR. Journal of Virological Methods. ,vol. 56, pp. 91- 98 ,(1996) , 10.1016/0166-0934(95)01958-8
D. J. F. Brown, W. M. Robertson, R. Neilson, F. Bem, D. J. Robinson, Characterization and vector relation of a serologically distinct isolate of tobacco rattle tobravirus (TRV) transmitted by Trichodorus similis in northern Greece European Journal of Plant Pathology. ,vol. 102, pp. 61- 68 ,(1996) , 10.1007/BF01877116
J.-L. Marty, B. Leca, T. Noguer, Biosensors for the detection of pesticides Analusis. ,vol. 26, pp. 144- 148 ,(1998) , 10.1051/ANALUSIS:199826060144
S Kintzios, E Pistola, P Panagiotopoulos, M Bomsel, N Alexandropoulos, F Bem, G Ekonomou, J Biselis, R Levin, Bioelectric recognition assay (BERA). Biosensors and Bioelectronics. ,vol. 16, pp. 325- 336 ,(2001) , 10.1016/S0956-5663(01)00127-0
David M. Rawson, Allison J. Willmer, Anthony P.P. Turner, Whole-cell biosensors for environmental monitoring Biosensors. ,vol. 4, pp. 299- 311 ,(1989) , 10.1016/0265-928X(89)80011-2