The subpopulation of microglia sensitive to neurotransmitters/neurohormones is modulated by stimulation with LPS, interferon-γ, and IL-4.

摘要: Recently, neurotransmitters/neurohormones have been identified as factors controlling the function of microglia, immune competent cells central nervous system. In this study, we compared responsiveness microglia to neurotransmitters/neurohormones. We freshly isolated from healthy adult C57Bl/6 mice and found that only a small fraction (1-20%) responded application endothelin, histamine, substance P, serotonin, galanin, somatostatin, angiotensin II, vasopressin, neurotensin, dopamine, or nicotine. cultured neonatal mice, similarly population these To induce proinflammatory phenotype, applied lipopolysaccaride (LPS) interferon-gamma (IFN-γ) cultures for 24 h. Several responding populations increased; however, there was no uniform pattern when comparing with LPS IFN-γ treatment. IL-4 an anti-inflammatory increased histamine-, P-, somatostatin-sensitive in adult, but not cells. also expression different receptors strongly correlated, indicating are many distinct set receptors. Our results demonstrate microglial heterogeneous respect their sensitivity they more responsive defined activation states.