Studies on degradation efficiency of polycaprolactone by a naturally-occurring bacterium.

摘要: We report a strain of Bacillus, isolated from the rhizosphere mangrove Sesuvium portulacastrum, that degrades polycaprolactone (PCL) on timescales are factor three shorter than hitherto reported, with complete degradation in only 20 days. The bacterium has been identified as Bacillus pumilus by means 16S rRNA gene sequencing and FAME analysis; it secretes proteases lipases its 'de-polymerase' activity is evident zone clearing emulsified PCL. It an aerobic chemoheterotroph capable utilizing variety carbohydrates. Although not true psychrophile, mesophile, growing optimally over temperature range 30-45 degrees C pH 5-12.5. halophile tolerating NaCI concentrations up to 10% w/v, unique degrading PCL monomer, epsilon-caprolactone (CL), sole carbon source. Degradation was monitored using Fourier Transform Infrared (FTIR) spectroscopy, light microscopy scanning electron (SEM). This found be enhanced salts (NaCl, KCI, MgSO4, Na2HPO4) at medium values excess 7. Under same growth conditions, another standard showed somewhat reduced PCL-degradation.