PDGF-BB released in tendon repair using a novel delivery system promotes cell proliferation and collagen remodeling
作者： Stavros Thomopoulos , Melissa Zaegel , Rosalina Das , Fredrick L. Harwood , Matthew J. Silva
DOI: 10.1002/JOR.20444
关键词:
摘要: The purpose of this study was to promote fibroblast proliferation and collagen remodeling in flexor tendon repair through sustained delivery platelet derived growth factor (PDGF-BB). release kinetics PDGF-BB from a novel fibrin matrix system initially evaluated vitro. After the vivo degradation rate determined using fluorescently tagged fibrin, delivered site canine model. effect on intrasynovial healing studied histology-based assays (cell density, proliferation, type I expression) by measuring total DNA levels reducible crosslink levels. provided vitro at modulated ratio heparin factor. In vivo, remained for more than 10 days. Delivery led qualitative increase cell mRNA expression. also statistically significant increases (20% 7 days, 18% 14 days) crosslinks (30% days). Sustained factors may be achieved fibrin-based system. increased thus accelerate healing.
sci-hub.se 参考文章(37)
James Chang, Richard Thunder, Daniel Most, Michael T. Longaker, William C. Lineaweaver, Studies in Flexor Tendon Wound Healing: Neutralizing Antibody to TGF-β1 Increases Postoperative Range of Motion Plastic and Reconstructive Surgery. ,vol. 105, pp. 148- 155 ,(2000) , 10.1097/00006534-200001000-00025
Curtis B. Herbert, George D. Bittner, Jeffrey A. Hubbell, Effects of fibrinolysis on neurite growth from dorsal root ganglia cultured in two‐ and three‐dimensional fibrin gels The Journal of Comparative Neurology. ,vol. 365, pp. 380- 391 ,(1996) , 10.1002/(SICI)1096-9861(19960212)365:3<380::AID-CNE4>3.0.CO;2-0
J.M. Verzijl, N., DeGroot, J., Ben-Zaken, C., Braun-Benjamin, O., Maroudas, A., Bank, R.A., Mizrahi, J., Schalkwijk, C.J., Thorpe, S.R., Baynes, J.W., Bijlsma, J.W.J., Lafeber, F.P.J.G., TeKoppele, Crosslinking by advanced glycation end products increases the stiffness of the collagen network in human articular cartilage: A possible mechanism through which age is a risk factor for osteoarthritis Arthritis & Rheumatism. ,vol. 46, pp. 114- 123 ,(2002) , 10.1002/1529-0131(200201)46:1<114::AID-ART10025>3.0.CO;2-P
S. Thomopoulos, G. Hattersley, V. Rosen, M. Mertens, L. Galatz, G. R. Williams, L. J. Soslowsky, The localized expression of extracellular matrix components in healing tendon insertion sites: an in situ hybridization study Journal of Orthopaedic Research. ,vol. 20, pp. 454- 463 ,(2002) , 10.1016/S0736-0266(01)00144-9
Andreas H. Zisch, Matthias P. Lutolf, Jeffrey A. Hubbell, Biopolymeric delivery matrices for angiogenic growth factors Cardiovascular Pathology. ,vol. 12, pp. 295- 310 ,(2003) , 10.1016/S1054-8807(03)00089-9
Steven C. Winters, Richard H. Gelberman, Savio L-Y. Woo, Serena S. Chan, Rupinder Grewal, John Gray Seiler, The effects of multiple-strand suture methods on the strength and excusion of repaired intrasynovial flexor tendons: A biomechanical study in dogs The Journal of Hand Surgery. ,vol. 23, pp. 97- 104 ,(1998) , 10.1016/S0363-5023(98)80096-8
Stavros Thomopoulos, Fred L. Harwood, Matthew J. Silva, David Amiel, Richard H. Gelberman, Effect of Several Growth Factors on Canine Flexor Tendon Fibroblast Proliferation and Collagen Synthesis In Vitro The Journal of Hand Surgery. ,vol. 30, pp. 441- 447 ,(2005) , 10.1016/J.JHSA.2004.12.006
Richard H. Gelberman, David Amiel, Fred Harwood, Genetic expression for type I procollagen in the early stages of flexor tendon healing. Journal of Hand Surgery (European Volume). ,vol. 17, pp. 551- 558 ,(1992) , 10.1016/0363-5023(92)90370-5
S. L. -Y. Woo, D. W. Smith, K. A. Hildebrand, J. A. Zeminski, L. A. Johnson, Engineering the healing of the rabbit medial collateral ligament Medical & Biological Engineering & Computing. ,vol. 36, pp. 359- 364 ,(1998) , 10.1007/BF02522484
Wafa M. Elbjeirami, Edward O. Yonter, Barry C. Starcher, Jennifer L. West, Enhancing mechanical properties of tissue-engineered constructs via lysyl oxidase crosslinking activity Journal of Biomedical Materials Research Part A. ,vol. 66, pp. 513- 521 ,(2003) , 10.1002/JBM.A.10021