Newly synthesized hepatocyte plasma membrane proteins are transported in transcytotic vesicles in the bile duct-ligated rat

摘要: Abstract Background: Newly synthesized apical membrane proteins in hepatocytes go first to the basolateral membrane, from which they are retrieved and delivered domain. The goal of present study was identify vesicular carriers these molecules. Methods: common bile duct rats ligated for 10–72 hours, then various plasma were localized using immunofluorescence quantitative immuno-electron microscopy fixed liver tissue. Results: By immunofluorescence, we found intracellular punctate staining near canalicular polymeric immunoglobulin A (IgA) receptor several proteins, but not proteins. This compartment bounded pleiomorphic by immunoelectron microscopy. Colocalization at electron microscopic level showed that protein, dipeptidyl peptidase IV, same structures as aminopeptidase N, IgA receptor, or intravenously injected horseradish peroxidase. immunolabeling decreased after cycloheximide treatment (t12 = 2–2.5 hours) reversal ligation 1 hour. In latter case, labeling increased. Furthermore, canaliculi. Conclusions: Bile leads an accumulation vesicles carrying a fluid phase marker. These continue fuse with even during ligation.