Preparation and characterization of universal Fe3O4@SiO2/CdTe nanocomposites for rapid and facile detection and separation of membrane proteins
作者： Min Wang , Ke-yan Zheng , Shao-wu Lv , Hai-feng Zou , Hong-sen Liu
DOI: 10.1039/C7NJ04484D
关键词:
摘要: Besides having many important physiological functions, cell membrane proteins have been recognized as major markers for diseases in clinical examination. The discovery of new has attracted significant research interest. Herein, a multifunctional Fe3O4@SiO2/CdTe nanocomposite was designed the detection and separation proteins, which synthesized by covalent coupling reaction between amino groups carboxyl groups. It can serve universal tool modifying antigens or other molecules that To verify feasibility practicability this method, folic acid (FA)-functionalized magnetic/fluorescent nanoprobe (Fe3O4@SiO2/CdTe-NH-FA) developed recognizing separating FA receptor on HepG2 cells. fixed to shell nanocomposites via 1,6-hexamethylenediamine. obviously reduces fluorescence quenching effect nanocomposites. results demonstrate Fe3O4@SiO2/CdTe-NH-FA provides excellent optical characteristics reliable imaging cancer cells good magnetic properties enrich isolate proteins. Consequently, biological be used find, obtain, study unknown predict occurrence some diseases. In addition, will potential platform accurate diagnosis therapy future.
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