Efficient delivery of Notch1 siRNA to SKOV3 cells by cationic cholesterol derivative-based liposome.

摘要: A novel cationic cholesterol derivative-based small interfering RNA (siRNA) interference strategy was suggested to inhibit Notch1 activation in SKOV3 cells for the gene therapy of ovarian cancer. The derivative, N-(cholesterylhemisuccinoyl-amino-3-propyl)-N, N-dimethylamine (DMAPA-chems) liposome, incubated with siRNA at different nitrogen-to-phosphate ratios form stabilized, near-spherical siRNA/DMAPA-chems nanoparticles sizes 100-200 nm and zeta potentials 40-50 mV. protected from nuclease degradation 25% fetal bovine serum. exhibited high cell uptake knockdown efficiency an ratio 100 concentration 50 nM. They also inhibited growth promoted apoptosis cells. These results may provide potential using derivatives as efficient nonviral carriers suppression cancer