Fast method for detection and/or identification of a single base in a nucleic acid sequence and its applications

摘要: The feature of this process is that: (1) the sequence on which base to be identified located made hybrid with a nucleotide adequate length; (2) synthesis complementary strand obtained in started, said acting as trigger, presence of: polymerase without 3'5' exonucleasis action; and at least one modified base; (3) incorporated blocking detected by any suitable means. Application: diagnosis genetic diseases.