PCR primer capable of reducing non-specific amplification and PCR method using the PCR primer

摘要: The present invention relates to a PCR primer facilitating hot-start by suppressing non-specific amplification at room temperature and the same time capable of reducing significantly dominating product rather than original template from third cycle, more precisely prepared additionally inserting reverse-complementary sequence certain region starting 5′-start site 5′-terminus which is composed priming anneal into method using same. has an additional sequence, inserted primer, suggesting that template-specific its are included in primer. can improve specificity amplification.