Method and system for more reliable determination of interaction parameters for low affinity analytes
作者： Karlsson Olof
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摘要: A method of determining one or more interaction parameters for the between an analyte and a ligand using biosensor, which comprises steps of: A: providing sensor surface having immobilized thereto, B: contacting with control analyte, C: registering response from binding to sites ligand, D: saturation (RmaxC) E: transforming (RmaxA) relative molar contribution analyte. F: samples containing different concentrations G: sites, H: fitting registered predetermined model determine parameters.
lens.org 参考文章(25)
Hugues Bedouelle, Elodie Brient-Litzler, Reagentless fluorescent biosensors comprising a designed ankyrin repeat protein module, rational design methods to create reagentless fluorescent biosensors and methods of their use ,(2009)
Caroline L. Schauer, David R. Walt, Frank J. Steemers, Cross-reactive sensors ,(2001)
Robert Karlsson, Markku Hamalainen, Stefan Lofas, Method and apparatus for assaying a drug candidate to estimate a pharmacokinetic parameter associated therewith ,(2000)
Olof Karlsson, Method of preparing supported lipid film membranes and use thereof ,(2002)
Jan Bergstrom, Bo Johnsson, Stefan Lofaas, Matrix coating for sensing surfaces capable of selective biomolecular interactions, to be used in biosensor systems ,(1994)
Eiichi Funatsu, Keiji Mabuchi, Masanori Kasai, Solid-state imaging device and drive control method for the same ,(2004)
Andrew P. Shreve, Karen M. Grace, Basil I. Swanson, Jennifer S. Martinez, Wynne K. Grace, Planar optical waveguide based sandwich assay sensors and processes for the detection of biological targets including protein markers, pathogens and cellular debris ,(2005)
Olavi Siiman, Method for the analysis of soluble analytes ,(2002)
Paola Borri, Adrian John Harwood, Trevor Clive Dale, Method of measuring the affinity of biomolecules ,(2006)
Xuedong Song, Diagnostic test kits with improved detection accuracy ,(2006)