A Single-Step Purification of the Glycoprotein of Nipah Virus Produced in Insect Cells using an Anion Exchange Chromatography Method
作者： Sunhare Raksha , Wen Siang Tan , Muhajir Hamid , Ramakrishnan Nagasundara Ramanan , Beng Ti Tey
DOI: 10.1080/01496395.2013.838265
关键词:
摘要: A single-step purification method based on an anion exchange chromatography was developed to purify truncated Nipah virus glycoprotein (tNiVG) expressed in Spodoptera frugiperda 9 (Sf9) insect cells. The preferred buffer conditions bind and elute tNiVG protein were 50 mM sodium carbonate with pH citrate 5, respectively. use of elution without chloride separated the from tightly bound major host proteins subsequently avoided desalting step as one further downstream processes. About 90% purity 89% recovery achieved method.
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