A role for PHANTASTICA in medio-lateral regulation of adaxial domain development in tomato and tobacco leaves

摘要: † Background and Aims Diverse leaf forms in nature can be categorized into two groups: simple compound. A has a single blade unit, whilst compound is dissected leaflets. For both leaves, MYB domain transcription factor PHANTASTICA (PHAN) plays an important role establishing the adaxial leaf. Absence of PHAN arabidopsis antirrhinum leaves supresses development, tomato suppresses leaflet development. However, rachis petiole regions where coexist, it been unclear why leaflets are not formed. We hypothesized that regulates medio-lateral extent domain, thereby determining architecture. Methods To test this hypothesis, we generated analysed transgenic plants expressing (SlPHAN) under Cauliflower mosaic virus (CaMV) 35S promoter sense antisense orientations, tobacco over-express SlPHAN. Key Results Modulations SlPHAN resulted variety morphologies such as simple, ternate either peltate or non-peltate arrangement. Measurements along wild-type axis showed narrowed comparison with laminar tissue arises. In antiSlPHAN no was formed medio-laterally narrowed, KNOX gene expression correlatively upregulated. CaMV35S::SlPHAN led to widening ectopic outgrowth tobacco. Taken together, these results suggest extension contributes final morphology tomato. Conclusions This study provides novel insight architecture highlights how changes master regulator translated diverse morphologies.