Virus-Induced Gene Silencing for Functional Genomics in Withania somnifera, an Important Indian Medicinal Plant
作者： Dikki Pedenla Bomzan , H. B. Shilpashree , P. Anjali , Sarma Rajeev Kumar , Dinesh A. Nagegowda
DOI: 10.1007/978-1-0716-0751-0_11
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摘要: Virus-induced gene silencing (VIGS) has emerged as a fast and efficient reverse forward genetics tool to study function in model plants well agriculturally important plants. In addition, VIGS approach been successfully used provide insights into the role of several genes regulators involved plant secondary metabolism. Ashwagandha (Withania somnifera) is an Indian medicinal that accumulates pharmacologically triterpenoid steroidal lactones, which are collectively termed withanolides. W. somnifera being highly recalcitrant for genetic transformation, Tobacco rattle virus (TRV)-mediated was established by our group facilitate understanding withanolides' pathway. Here, we describe detailed procedure carry out studies somnifera.
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