A collagen-binding EGFR single-chain Fv antibody fragment for the targeted cancer therapy
作者： Hui Liang , Xiaoran Li , Bing Chen , Bin Wang , Yannan Zhao
DOI: 10.1016/J.JCONREL.2015.04.029
关键词:
摘要: Collagen, a primary component of the extracellular matrix (ECM), is highly expressed in variety cancers and influences tumor microenvironment by increasing recruitment macrophages endothelial cells. Therefore, collagen promising target for cancer therapy. The collagen-binding domain (CBD) can dynamically bind to achieve sustained release CBD-fused protein network. Here, we developed epidermal growth factor receptor (EGFR) antibody fragment targeting collagen-rich ECM tumors. single chain variable (scFv) cetuximab was fused CBD (CBD-scFv) Pichia pastoris. CBD-scFv preserved antigen binding anti-tumor activity vitro. Moreover, displayed ability due function CBD. In vivo experiments revealed that bound achieved Furthermore, significantly suppressed tumors A431 xenografts. had potential therapeutic value carcinomas. specific could be new approach targeted drug delivery
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