Expression analysis of mouse Rhobtb3 using a LacZ reporter and preliminary characterization of a knockout strain.

摘要: RhoBTB3 is an atypical member of the Rho family small GTPases. It localizes at Golgi apparatus and endosomes involved in vesicle trafficking targeting proteins for degradation proteasome. Previous studies using Northern blot analysis showed that Rhobtb3 ubiquitously expressed adult mice, but expression particularly high brain, heart uterus. The gene also between embryonic days 11.5 17.5. To investigate specific cell types express this across tissues, both embryo organism, we have made use a trap mouse strain expresses LacZ under transcriptional control endogenous promoter. Histochemical detection β-galactosidase revealed profile characterized by nearly ubiquitous embryo, with levels bone, cartilage, all muscle, testis restricted areas nervous system. In adult, persists much lower cardiac tunica media blood vessels cartilage seminiferous tubules. A general preliminary characterization reduced viability, postnatal growth defect size. Our results should pave way future aimed investigating roles tissue development cardiac, vascular testicular function.