Heterologous expression of cellobiohydrolases in filamentous fungi – An update on the current challenges, achievements and perspectives

摘要: Abstract Cellobiohydrolases are among the most important enzymes functioning in hydrolysis of crystalline cellulose, significantly contributing to efficient biorefining recalcitrant lignocellulosic biomass into biofuels and bio-based products. Filamentous fungi recognized as both well-known producers commercial preparations cellulolytic hosts for heterologous protein secretion. Thus, Aspergillus Trichoderma species have been chosen expression native or engineered aiming at overproduction single secretion multi-enzyme cocktails on-site production biorefineries, which is reducing costs conversion. An even more interesting aspect consolidated bioprocessing, a fungus hydrolyzes lignocellulose polymers ferments resulting sugars valuable However, due low cellobiohydrolase activities, certain might be deficient with regard value cellulose conversion, improving filamentous has proven challenging. In this review, we examine effects altering promoters, signal peptides, culture conditions host post-translational modifications. For become an industrially feasible process, construction site-integrating plasmids, development protease-deficient strains glycosylation engineering obvious targets constructing enzyme producers.