Leaf-inhabiting endophytic fungi of European Beech ( Fagus sylvatica L.) co-occur in leaf litter but are rare on decaying wood of the same host

摘要: Many microfungi are able to live in living plant tissues. In contrast pathogens and parasites the so-called endophytic fungi do not cause obvious disease symptoms their hosts. Nevertheless, they constitute an ubiquitous active component direct multitrophic interactions. The present study was conducted assess level of overlap cultivable decaying tissues European Beech (Fagus sylvatica L.) from a forest stand North-Eastern Germany. focus lay on hypothesized fall-spring relationship leaf-inhabiting endophytes, which means that endophytes autumn leaves persist as saprobes litter or dead wood, sporulate re-invade spring. Fungal cultures were isolated leaves, leaf wood still attached tree by dilution-to-extinction cultivation years 2007–2010. Analyses species identity, richness composition based microscopic identification sequencing fungal DNA ‘barcode’ ITS (internal transcribed spacer). Species litter-inhabiting equaled wood-inhabiting exceeded endophytes. most distinctive assemblage observed also significantly different each other. On other hand considerable compositional phylogenetic between revealed with phylogenetics, cluster analysis non-metric multidimensional scaling. taxa accounting similarity belonged Capnodiales, Xylariales, Diaporthales Pleosporales. Finally, data cultivated beech compared 454 sequence set phyllosphere. This allowed partition lists into “epiphytes” dormant propagules.