Recombinant Cytomegaloviruses for Study of Replication and Pathogenesis

摘要: Systematic mutagenesis of large viral genomes such as those the cytomegaloviruses requires strategies for identifying relevant functions well detailed analysis particular genes. A number genetic markers that have been developed in other biological systems useful insertion these viruses. Thus far, 57 over 227 genes carried by wild-type human cytomegalovirus found to be dispensable growth cultured cells. Because limitations on studying an animal host, closely related murine has used a surrogate pathogenesis, tissue tropism and latency studies laboratory mouse. Genetic this virus paralleled work cytomegalovirus, understanding specifically impact organs emerged from studies. Strategies generation permissive cell lines able complement essential replication described, sets cosmid clones generate recombinant These methods will enable systematic functional cytomegaloviruses.