A general method for maximizing the expression of a cloned gene.

摘要: Abstract We present a method, utilizing combination of restriction endonuclease cleavage and digestion with Escherichia coli exonuclease III Aspergillus orizae nuclease S1, that allows us to position fragment bearing the promoter lacZ gene E. at virtually any distance in front cloned gene. In particular, we have used this method examine effect on protein production gene-promoter separation for cro phage lambda produce plasmids that, upon transformation into appropriate hosts, direct synthesis up 190,000 monomers per cell.