Hybrid-primed lymphocytes and hybrid vaccination prevent tumor growth of lewis lung carcinoma in mice.

摘要: Dendritic cell (DC)-tumor hybrids are currently being evaluated as a novel antitumor vaccination strategy. We have explored in an animal model whether administration of DCs fused with poorly immunogenic carcinoma cells could elicit response. Fusion C57/BL6 mice bone marrow-derived Lewis lung (LLC1) resulted approximately 50% fusion efficiency. Hybrid (HCs) were used to explore 3 potential tumor therapy strategies: protective immunization, vaccination, and adoptive cellular therapy. Immunization HCs induced activation proliferating cytotoxic T cells, upregulation distinct cytokines genes, significant retardation growth. Similar results observed by the tumor-bearing host. Finally, when from HC-vaccinated transferred into naive mice, growth was strongly retarded efficient proliferative T-cell response observed. Tumor reduced more than 50%, development significantly delayed. Taken together, we demonstrate that offer effective immunotherapy carcinomas. This is independent taken for transfer or vaccine.