Regulation of chemokine gene expression and secretion in Staphylococcus aureus-infected osteoblasts.

摘要: Abstract Staphylococcus aureus is the major cause of osteomyelitis or bone infection, leading to morbidity, often in children. Little known about immunopathogenesis osteomyelitis, although uncontrolled inflammation a clinical feature. This study investigated effects dexamethasone, PGE 2 and T h 2 cytokines, all potential down-regulatory mediators, on control S. -induced C-X-C (CXCL8, CXCL10) C-C (CCL5, CCL2) chemokine gene expression secretion from human osteoblastic MG-63 cells primary NHOst cells. Chemokine mRNA were reduced 50–75% by whereas doubled accumulation, as detected RNase protection assay RT-PCR, but decreased 33–71% ( P osteoblast secretion, IL-4 CXCL8 2.5-fold increased CXCL10 3-fold (all 2 cytokines infection osteoblasts, dexamethasone have diverse, sometime upregulatory actions chemokines due both pre- post-transcriptional secretion.