Ambiguous phenotypes and genotypes in 16 children with acute leukemia as characterized by multiparameter analysis.

摘要: Ambiguous phenotypes and genotypes were observed in 16 children with acute leukemia. Surface marker, cytogenetic, molecular genetic, DNA flow cytometric analyses as well standard morphologic cytochemical studies used to divide the patients into three groups. The first group comprised five leukemia whose blast cells morphologically lymphoid, while immunophenotyping disclosed simultaneous expression of early pre-B cell myeloid features. Molecular genetic showed evidence heavy-chain immunoglobulin (Ig) gene rearrangements all patients. Cytogenetic data, available these children, revealed t(4;11). In patients, surface marker indicated coexistence two separate populations, one other Further B commitment was provided by demonstration Ig Surprisingly, oligoclonal monoclonal rearrangement for beta chain T receptor (beta-TCR). last consisted four cases otherwise typical lymphoblastic (ALL), phenotype, coexpression or cell-associated antigens, unequivocal (AML) antigens. Gene could be demonstrated six additional light-chain ALL, bigenotypic features (Ig beta-TCR rearrangement) patient. none did cytometry disclose clonal abnormalities leukemic content. Based on findings, we suggest that malignant transformation second took place at a stage ontogenetically close pluripotent stem cell, whereas ambiguous third resulted from aberrant insufficient reagent specificity.