Cas3 is a single‐stranded DNA nuclease and ATP‐dependent helicase in the CRISPR/Cas immune system

作者: Tomas Sinkunas , Giedrius Gasiunas , Christophe Fremaux , Rodolphe Barrangou , Philippe Horvath

DOI: 10.1038/EMBOJ.2011.41

关键词:

摘要: … Cas3 possesses a single‐stranded DNA (ssDNA)‐stimulated ATPase activity, which is … in the HD domain with a preference for ssDNA substrates. To dissect the contribution of individual …

参考文章(37)
Manel Camps, Lawrence A Loeb, Critical role of R-loops in processing replication blocks. Frontiers in Bioscience. ,vol. 10, pp. 689- 698 ,(2005) , 10.2741/1564
Moonjung Hyun, Vilhelm A. Bohr, Byungchan Ahn, Biochemical characterization of the WRN-1 RecQ helicase of Caenorhabditis elegans. Biochemistry. ,vol. 47, pp. 7583- 7593 ,(2008) , 10.1021/BI800197M
Rotem Sorek, Victor Kunin, Philip Hugenholtz, CRISPR — a widespread system that provides acquired resistance against phages in bacteria and archaea Nature Reviews Microbiology. ,vol. 6, pp. 181- 186 ,(2008) , 10.1038/NRMICRO1793
Josiane E. Garneau, Marie-Ève Dupuis, Manuela Villion, Dennis A. Romero, Rodolphe Barrangou, Patrick Boyaval, Christophe Fremaux, Philippe Horvath, Alfonso H. Magadán, Sylvain Moineau, The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA. Nature. ,vol. 468, pp. 67- 71 ,(2010) , 10.1038/NATURE09523
Francisco J.M. Mojica, Chc)sar D�ez-Villase�or, Jes�s Garc�a-Mart�nez, Elena Soria, Intervening Sequences of Regularly Spaced Prokaryotic Repeats Derive from Foreign Genetic Elements Journal of Molecular Evolution. ,vol. 60, pp. 174- 182 ,(2005) , 10.1007/S00239-004-0046-3
Stanley C. Gill, Peter H. von Hippel, Calculation of protein extinction coefficients from amino acid sequence data Analytical Biochemistry. ,vol. 182, pp. 319- 326 ,(1989) , 10.1016/0003-2697(89)90602-7
Tapan Biswas, Jessica M. Pero, Caleb G. Joseph, Oleg V. Tsodikov, DNA-dependent ATPase activity of bacterial XPB helicases. Biochemistry. ,vol. 48, pp. 2839- 2848 ,(2009) , 10.1021/BI8022416
S. J. J. Brouns, M. M. Jore, M. Lundgren, E. R. Westra, R. J. H. Slijkhuis, A. P. L. Snijders, M. J. Dickman, K. S. Makarova, E. V. Koonin, J. van der Oost, Small CRISPR RNAs guide antiviral defense in prokaryotes Science. ,vol. 321, pp. 960- 964 ,(2008) , 10.1126/SCIENCE.1159689
Olivier Cordin, Josette Banroques, N. Kyle Tanner, Patrick Linder, The DEAD-box protein family of RNA helicases Gene. ,vol. 367, pp. 17- 37 ,(2006) , 10.1016/J.GENE.2005.10.019