Molecular Epidemiology of Rotaviruses in Nigeria: Detection of Unusual Strains with G2P[6] and G8P[1] Specificities

摘要: During an epidemiological study on rotaviruses among diarrheic children in the northeastern and middle belt regions of Nigeria, distribution G P types was investigated 127 stool specimens. By PCR typing, type 97 samples identified. Interestingly, unusual G8 type, as well common G1, G2, G3 types, detected more frequently (31 112; 27.7%). Eleven contained multiple a G9 strain (Bulumkutu) identified for one probable mixed infections. In P[6] most frequently, P[8] being second while 73 could not be One rotavirus with specificity cultivated cell culture, this found to P[1], which is usually carried by bovine strains. When combinations were examined, strains G2P[6] G8P[1] often Sequence analysis performed VP7 gene Bulumkutu VP4 genes HMG035. The sequence Nigerian serotype closely related that Brazilian than those other African HMG035 very similar Thai A5, suggesting may have been transmitted directly humans. These results highlight unexpected diversity Nigeria emphasize need further serological genetic surveys countries, including Nigeria.