An ERG (ets-related gene)-associated histone methyltransferase interacts with histone deacetylases 1/2 and transcription co-repressors mSin3A/B.

摘要: Covalent modifications of histone tails play important roles in gene transcription and silencing. We recently identified an ERG ( ets -related gene)-associated protein with a SET (suppressor variegation, enhancer zest trithorax) domain (ESET) that was found to have the activity H3-specific methyltransferase. In present study, we investigated interaction ESET other chromatin remodelling factors. show methyltransferase associates deacetylase 1 (HDAC1) HDAC2, also interacts co-repressors mSin3A mSin3B. Deletion analysis reveals N-terminal region containing tudor is responsible for mSin3A/B association HDAC1/2, truncation enhances its binding mSin3. When bound promoter, represses downstream luciferase reporter gene. This repression by independent activity, but correlates mSin3 co-repressors. addition, can be partially reversed treatment HDAC inhibitor trichostatin A. Taken together, these data suggest form large, multi-protein complex(es) HDAC1/2 participates multiple pathways transcriptional repression.