Effects of Nicotine on the Number and Activity of Circulating Endothelial Progenitor Cells

摘要: Recently, some studies have shown that nicotine increased neovascularization, which involves endothelial progenitor cells (EPCs). The effects of on EPCs are still unclear at present. Therefore, the authors investigated whether had influences EPC number and activity. were stimulated with (to make a series final concentrations: 10 - 1 2 mol/L, 0 8 6 4 mol/L) or vehicle control for respective time points(12, 18, 24, 32, 48 hours). characterized as adherent double positive DiLDL uptake lectin binding by direct fluorescent staining under laser-scanning confocal microscope. They further documented demonstrating expression KDR, VECFR-2, AC133 flow cytometry. proliferation, migration, in vitro vasculogenesis activity assayed 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay; modified Boyden chamber kit, respectively. adhesion assay was performed replating those fibronectin-coated dishes then counting cells. As result, dose dependently proliferative, migratory, adhesive, capacity concentrations to mol/L. peak observed similar blood smokers. In addition, (10 However, cytotoxicity seen higher (> mol/L). conclusion, complex EPCs: might induce augmentation enhanced functional relatively low concentrations.