Complex regulation of acetylcholinesterase gene expression in human brain tumors.

摘要: To study the regulation of acetylcholinesterase (AChE) gene expression in human brain tumors, 3' splice variants AChE mRNA and potentially relevant transcription factor mRNAs were labeled primary astrocytomas melanomas. AChE-S AChE-R mRNA, as well Runx1/AML1 accumulated correlation with tumor aggressiveness, but neither HNF3beta nor c-fos was observed melanoma astrocytomas. Immunohistochemistry demonstrated nuclear cellular melanomas, however, only AChE-S, not secreted variant, retained astrocyte cells. revealed weak linkage ACHE promoter sequences, yet enhanced co-transfected COS1 The p300 co-activator promoter's distal enhancer facilitated this effect, which independent much trans-activation domain. Surprisingly, GASP, a fusion product green fluorescence protein (GFP) ASP(67), peptide composed 67 C-terminal amino acid residues localized to cell nuclei. However, GARP, corresponding GFP having 51 AChE-E or alone, remained cytoplasmic. exhibited improved retention GASP-expressing cells, suggesting modulated localization processes. Together, these findings reveal tumor-specific both variant products.