Antiproliferative Effects of Gastrin Receptor Antagonists and Antibodies to Gastrin on Human Colon Carcinoma Cell Lines

摘要: The gastrointestinal hormone gastrin has been shown to stimulate the growth of normal colonic mucosa. To examine for a possible role in proliferation cultured colon tumor cells, we have studied effects two receptor antagonists, proglumide and benzotript, antibodies gastrin. We find that (50% effective concentration, 2 5 mm) benzotript 0.4 0.8 inhibit monolayer six human cancer cell lines. Addition exogenous abrogated growth-inhibitory effect proglumide. anchorage-independent carcinoma cells was also inhibited by antagonists. Also, dose-dependent increase carcinoembryonic antigen secretion observed upon treatment with three lines examined. Half-maximal inhibition labeled binding at concentrations mm 8.6 In addition, antigastrin antiserum added HCT 116 adapted serum-free medium resulted concentration-dependent cellular proliferation. These data suggest may function as an autocrine factor carcinoma.