Exploring potential of i-motif DNA formed in the promoter region of GRIN1 gene for nanotechnological applications
作者： Swati Chaudhary , Mahima Kaushik , Saami Ahmed , Shrikant Kukreti , None
DOI: 10.1016/J.RECHEM.2020.100086
关键词:
摘要: Abstract The tandem repeat stretches of cytosine residues fold to construct intercalated motif (i-motif) structures over an acidic pH range. Several advancements have been made understand the biological role i-motif DNA. Potential found in gene promoters are involved positive transcriptional regulation and sequence specific DNA binding. Herein, a 27-mer C-rich stretch present at promoter region (Glutamate Ionotropic Receptor NMDA type subunit 1) GRIN1 gene, named GRIN1c was studied investigate its structural status using biophysical biochemical techniques. gel electrophoresis, circular dichroism spectroscopy pH-dependent monophasic UV-thermal melting data revealed that adapts bimolecular structure. Two alternative models suggested for structure with typical C·C+ base pairing significant Watson-Crick pairing. Possibility co-existence non-Watson-Crick formation expands repertoire various topologies structures. Extensive applications areas nanotechnology, analytical biomedical systems many more it essential explore these multistranded Nanodevices-based on as biosensors drug release platform, also serve proof their vivo existence.
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