Molecular detection of cell line cross-contaminations using amplified fragment length polymorphism DNA fingerprinting technology
作者： E. Milanesi , P. Ajmone-Marsan , E. Bignotti , M. N. Losio , J. Bernardi
DOI: 10.1007/S11626-003-0006-Z
关键词:
摘要: We have tested amplified fragment length polymorphism (AFLP) technology, in comparison with isoenzyme analysis, for the simultaneous detection of inter- and intraspecific cell line cross-contaminations (CCCs) collection held at Istituto Zooprofilattico della Lombardia e dell'Emilia Romagna. Isoenzyme analysis identified four cases interspecific CCCs. In a single experiment, AFLP was able to identify species origin all lines which reference genomic deoxyribonucleic acid available detect five contaminations. Four CCCs confirmed data on isoenzymes, whereas fifth CCC detected noninformative. addition, putative source contaminations detected. The utility technology depends number that be distinguished specific availability highly informative fingerprinting systems. mice, primer pair produced 16 polymorphisms 15 strains mouse analyzed. humans, 18 AFLPs 83 different profiles 159 Amplified can conveniently applied hypervariable markers are not available. multiplex microsatellite is available, still provide useful cheap tool simultaneously testing
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