Evaluation of transport and storage techniques for isolation of Campylobacter fetus subsp. jejuni from turkey cecal specimens.

摘要: Immediate culturing of fecal specimens is not always possible, and appropriate methods for transport storage Campylobacter fetus subsp. jejuni have been fully evaluated. Using nine techniques, we studied the survival C. in cecal from infected turkeys. The organisms survived held without medium 3 to 15 days (median, 9 days) at 4 degrees C, 2 25 Only 20% frozen 24 h either -20 or -70 C yielded jejuni. Specimens dried on filter paper strips were negative within 1.5 h. Cary-Blair with decreased agar was best six media tested, it enabled recovery organism 100% (3 71% (7 samples 94% 85% In contrast, more than half all Culturettes buffered glycerol saline by days, 7 days. Results other three (Campy-thio, thioglycolate medium, alkaline peptone water) intermediate. Overnight incubation water 37 42 did enhance Therefore, refrigeration a satisfactory up specimens, however, recommend use that must be transported stored longer rectal swabs, prevent drying.