In interleukin-4-deficient mice, alum not only generates T helper 1 responses equivalent to freund's complete adjuvant, but continues to induce T helper 2 cytokine production.

摘要: The role of interleukin (IL)-4 in the activity two frequently used vaccine adjuvants, Freund's complete adjuvant (FCA) and aluminum hydroxide gels (alum), was studied using standard antigen ovalbumin (OVA) IL-4 genedisrupted mice (IL-4 -/-). In absence adjuvant, there an overall reduction antibody production to OVA -/- significantly greater amounts interferon (IFN)-gamma were produced following restimulation splenocytes with vitro compared immunocompetent controls +/+). FCA alum boosted immune response both +/+ mice. mice, while stimulated a wide-spectrum immunoglobulin response, including Th1-associated IgG2a Th2-associated IgG1, enhanced only Th2 no OVA-specific could be detected. IL-4-deficient however, not increased all adjuvant-treated groups, but as potent at stimulating this subclass FCA. Similarly, by Th1 cytokine IFN-gamma, equivalent that after inoculation FCA/OVA, detected inoculated alum/OVA. There IgE IgG1 production, although still significant levels, reduced wild-type irrespective used. However, IL-5 totally inhibited difference between strains when adjuvant.